there's one I get many opportunities to say-: "oh, you're no fun anymore"
I also have a few of the quotes with actual sound links to them set as sounds on my computer. For example, when I log in, it says "this is an ex-parrot!". When a warning comes up (something finishes downloading), it either says "oh bloody hell," or "(twang) message for you, sir." Finally, when I log off, it says "how shall we fuck off, O Lord?"
Surprisingly, many of my friends are Python/ Tom Lehrer fans.