Alas, the Record Lady's web site is gone, and no one here ever posted Acuff's lyrics. Fortunately, there are many commercial recordings available, and consequently many sound samples at, for example, allmusic. I listened to several of them. Acuff himself made several recordings. The instrumental arrangements vary considerably, but the lyrics are fairly consistent. I think the following composite is a good representation. I have italicized the differences between Acuff's recordings and the Cathy Fink/Marcy Marxer version that rich-joy posted above:

FREIGHT TRAIN BLUES
Roy Acuff/Traditional

I was born in Dixie in a boomer's shack,
Just a little shanty by a railroad track.
The humming of the drivers was my lullaby.
An' a freight train whistle taught me how to cry.

CHORUS: I got the freight-train blues.
Lordy, Lordy, Lordy! Got 'em in the bottom of my ramblin' shoes.
And when the whistle blows, I gotta go.
Oh, Lordy, guess I'm never gonna lose the freight-train blues.

My daddy was a-farmin' an' my mama dear
She was the only daughter of an engineer.
Sweetie loved a brakeman. Now, it ain't no joke,
But it's a shame the way she keeps a good man broke.

A steamboat whistle never stirs my soul.
Aeroplanes an' autos always leave me cold.
The only thing that makes me want to navigate
Is the wildcat whistle on a southbound freight.

I know I'm old enough to quit my running around.
I've tried a hunnerd times to stop an' settle down,
But ever' time I find a place I want to stay,
I hear a freight-train whistle, Lord, I'm on my way.