Just watched it again. I'm not sure his guitar was out of tune - I noticed that, as the opening shot panned from JH to JT, he appeared to be tweaking a tuner-key. He also had a Snark on the headstock, so he certainly had the means to do a last-few-seconds tuning check. I have a suspicion it was the FX he used that gave that 'out of tune' warbling sound, along with the fact that he was in a low tuning which can play havoc with pitch, esp. on the 6th string. Not sure what FX he was using, could have been maybe Tremolo or Chorus, or both?
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