You can find a recording of this Dixieland song to listen to if you like but here are the lyrics as I've patiently tried to transcribe them:

As led by John Gordy (1904-1961) with Hots Jackson & The Old-Timers band, ©1952

Steamboat, Blow Your Whistle Again

Steamboat, laid low again,
Black smoke, around the bend,
Steaming down the river past a million scenes,
Loaded down with cotton bales bound for New Orleans;
Steamboat, where have you gone?
Where is your lonesome song?
Like a mighty organ plays an old refrain
Your whistle blew away my cares an’ lonely pain;
Steamboat, blow your whistle again!

(Musical interlude)

Steamboat, please go again,
Black smoke, around the bend,
Steaming down the river past a million scenes,
Loaded down with cotton bales for New Orleans;
Steamboat, where have you gone?
Where is your lonesome song?
Like a mighty organ plays an old refrain
Your whistle blew away my cares an’ lonely pain;
Steamboat, blow your whistle again!

(Musical interlude)

Steamboat, where you gone?
Where is your lonesome song?
Like a mighty organ plays an old refrain
Your whistle blew away my cares an’ lonely pain;
Steamboat, blow your whistle, blow your whistle, blow your whistle again!

Cheerily,
Charlie Ipcar