In those days Bob's guitar tended to have old strings that were pretty dead on it much of the time. Such strings are hard to tune, period. On top of that, he was seemingly sometimes a bit careless about it. The electric guitar on the recording of "Queen Jane Approximately" in 1965 is considerably out of tune (it's flat). It's so notable that it may even have been deliberate. One thing about playing guitar and harmonica...if the guitar is out of tune it clashes badly with the harmonicas and you notice right away. Most of Bob's stuff sounded pretty much in tune back then, but now and then it wasn't. As for this particular recording, well, I'll have to listen to it again to see about that.

We are all occasionally challenged when it comes to tuning a guitar onstage. :-)

- LH