Lyr. Add: IT'S AWFUL SAD WHEN THE HOLY GHOST IS GONE

You can play the ace and tray,
But it sho' will lead you astray.
It's awful sad when the Holy Ghost is gone.

You can weep and you can moan,
But hell goin' be yo' home.
It's awful sad when the Holy Ghost is gone.

You can play the ace and deuce,
But God'll catch you with no excuse.
It's awful sad when the Holy Ghost is gone.

You can pass the whiskey round,
But you must lay yo' body down.
It's awful sad when the Holy Ghost is gone.

Sung by Ed Lloyd, NC; MS of N. I. White 1925. Also collected 1919 by W. B. Jeffrey, but each stanza is 'given gravy' by adding 'um-m, umm-m' at the end of each verse.
N. I. White, 1928, "American Negro Folk Songs," Religious Songs No. 18A, p. 78.

See THE GAMBLER (2) in the DT, from Louise Pound, "American Ballads and Songs."
(One verse given here for comparison:

My father was a gambler, he learnt me how to play, (2x)
Saying, 'Son, don't go a-begging when you hold the ace and tray,
When you hold the ace and tray.'
Singing of Minnie Dowd, coll. Frances Barbour 1917, Arlington, MO.
H. M. Belden, 1940, Ballads and Songs, p. 472.
Not African-American and not religious, but 'ace and tray' mentioned. Probably not related.

Lyr. Add: (OH, CRAP-SHOOTER)
Untitled, No. 17 of Religious Songs.

Oh, crap-shooter, Oh, crap-shooter
You can shoot all ober dis world,
You can shoot from de six, from de seven, to de 'leben,
But God knows you can't shoot er inter heben.

Oh, back-biter, Oh, back-biter,
You can bite all ober dis world,
You can bite from de six, to seben, to de 'leben,
But God knows you can't shoot er into heben.

Oh, schemer, Oh, schemer,
You can scheme all ober dis world,
You can scheme from de six, to seben, to de 'leben,
But God knows you can't shoot er inter heben.

MS of A. H. Williamson, Alabama, coll. 1915-1916.
No. 17, Religious Songs, p. 77; N. I. White, 1928, "American Negro Folk-Songs."

More verses?
More songs that fit the thread title?

(I remember card players who would not call the bet if they held aces and trays).